Infectious diseases in 2,000-year-old faeces from the Silk Road
Once travelled by famous historical figures such as Marco Polo and Genghis Khan, the Silk Road was a hugely important network of transport routes connecting eastern China with Central Asia, the Middle East and Europe. It came to prominence during the Chinese Han Dynasty (202 BC to AD 220) and remained a key transport route for the following 2,000 years.
Given that the Silk Road was a melting pot of people, it is no wonder that researchers have suggested that it might have been responsible for the spread of diseases such as bubonic plague, anthrax and leprosy between China and Europe. However, no one one has yet found any evidence to show how diseases in eastern China reached Europe. Travellers might have spread these diseases taking a southerly route via India and the Middle East, or a northerly route via Mongolia and Russia.
But our team, including researchers at the University of Cambridge and China’s Academy of Social Sciences and Gansu Institute for Cultural Relics and Archaeology, has now found the earliest evidence for the spread of infectious disease organisms along the Silk Road. The results have been published in the Journal of Archaeological Science Reports.
Faeces under the microscope
We realised that this material was faeces when we looked at it with a high-powered optical microscope. We also found the eggs of four species of parasitic intestinal worms in it. This may seem surprising but the eggs of many species of intestinal worms are very tough and may survive thousands of years in the ground. This indicates that some of the people using this latrine 2,000 years ago were infected with parasites. The species included roundworm (Ascaris lumbricoides), whipworm (Trichuris trichiura), Taenia sp. tapeworm (likely T. solium, T. asiatica or T. solium) and Chinese liver fluke (Clonorchis sinensis).
Discovering evidence for Chinese liver fluke at a latrine in the arid region of Dunhuang was really exciting. The parasite could not possibly be endemic in that region as there are no marshy areas needed for its life cycle. Instead, it shows that a person who became infected with the liver fluke in eastern or southern China was able to travel the huge distance to this relay station along the Silk Road – at least 1,500km.
Our finding suggests that we now know for sure that the Silk Road was responsible for spreading infectious diseases in ancient times. This makes more likely previous proposals that bubonic plague, leprosy and anthrax could also have been spread along it.